Journal:

Article Title: Identification of an Abscisic Acid Gene Cluster in the Grey Mold Botrytis cinerea

doi: 10.1128/AEM.02919-05

Figure Lengend Snippet: Postulated biosynthetic pathway of ABA in C. pini-densiflorae (late steps, modified from a method described previously by Okamoto et al. [34]). Bold lines indicate the major route. Intermediates identified are α-ionylideneacetic acid (3), 4′-S-OH-α-ionylideneethanol (4), 1′-OH-α-ionylideneethanol (5), 4′-R-OH-α-ionylideneacetic acid (6), 4′-S-OH-α-ionylideneacetic acid (7), and 1′-OH-α-ionylideneacetic acid (8). The enzymatic steps possibly catalyzed by BcABA1, BcABA2, and BcABA4 are indicated.

Article Snippet: All intergenic regions were analyzed with the exon-trapping software program Xpound ( http://bioweb.pasteur.fr/-seqanal/interfaces/-xpound-simple.html ), revealing that the probability of finding more genes in the investigated sequence is low (data not shown). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Gene Length (bp) Intron(s) (bp) Encoded polypeptide (aa) Function (putative) bcorf2 Ferulic acid esterase bcaba3 1,254 417 bcaba1 1,769 48, 49, 83, 59 509 P450 monooxygenase bcaba2 1,771/1,810 50, 66, 50, 60 514/527 P450 monooxygenase bcaba4 842 65 258 Short-chain dehydrogenase/ reductase bcpl1 Fungal pectin lyase Open in a separate window Characteristics of genes located adjacent to bcaba1 Because the genomic DNA library used in these studies was derived from strain SAS56, which probably does not produce abscisic acid in axenic culture, we wanted to test whether the organization of the sequenced genes is the same in the nonsporulating ABA overproducer ATCC 58025 and the highly pathogenic strain B05.10 used in most molecular studies.

Techniques: Modification

Journal:

Article Title: Identification of an Abscisic Acid Gene Cluster in the Grey Mold Botrytis cinerea

doi: 10.1128/AEM.02919-05

Figure Lengend Snippet: Characteristics of genes located adjacent to bcaba1

Article Snippet: All intergenic regions were analyzed with the exon-trapping software program Xpound ( http://bioweb.pasteur.fr/-seqanal/interfaces/-xpound-simple.html ), revealing that the probability of finding more genes in the investigated sequence is low (data not shown). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Gene Length (bp) Intron(s) (bp) Encoded polypeptide (aa) Function (putative) bcorf2 Ferulic acid esterase bcaba3 1,254 417 bcaba1 1,769 48, 49, 83, 59 509 P450 monooxygenase bcaba2 1,771/1,810 50, 66, 50, 60 514/527 P450 monooxygenase bcaba4 842 65 258 Short-chain dehydrogenase/ reductase bcpl1 Fungal pectin lyase Open in a separate window Characteristics of genes located adjacent to bcaba1 Because the genomic DNA library used in these studies was derived from strain SAS56, which probably does not produce abscisic acid in axenic culture, we wanted to test whether the organization of the sequenced genes is the same in the nonsporulating ABA overproducer ATCC 58025 and the highly pathogenic strain B05.10 used in most molecular studies.

Techniques:

Journal: eLife

Article Title: The role of structural pleiotropy and regulatory evolution in the retention of heteromers of paralogs

doi: 10.7554/eLife.46754

Figure Lengend Snippet:

Article Snippet: The reactions were incubated overnight at 25°C and inactivated with proteinase K. We used the whole reaction to transform MC1061 competent E. coli cells, followed by selection on solid 2YT medium supplemented with 100 mg/L ampicillin (BioShop Inc, Canada) at 37°C.

Techniques: Recombinant, Plasmid Preparation, Sequencing, Software